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An immersed hollow fiber membrane bioreactor for enhanced biotransformation of indene to cis-indandiol using Pseudomonas putida

Paper ID Volume ID Publish Year Pages File Format Full-Text
3201 157 2014 7 PDF Available
Title
An immersed hollow fiber membrane bioreactor for enhanced biotransformation of indene to cis-indandiol using Pseudomonas putida
Abstract

•We developed an immersed membrane bioreactor for pharmaceuticals biotransformation.•System showed enhanced cis-indandiol conversion from indene.•Optimized operation increased product concentration by 4.5 times compared to suspension cultures.•Membranes served to immobilize cells and sequester inhibitory indene.•Reusability of membranes demonstrated over 5 repeated cycles.

An immersed hollow fiber membrane bioreactor (IHFMB) was fabricated for production of pharmaceutical intermediates cis-indandiol from indene by Pseudomonas putida ATCC 55687. At 2–6 g/L indene, significant increase of 160–400%, corresponding to 240–400 mg/L of cis-indandiol was achieved as compared to that obtained from suspension culture bioreactors (SCB). The IHFMB volumetric productivity of 10.1–12.9 mg/(L h) and molar yield of 0.050–0.093 mol/mol indene was also higher than the 4.3–8.3 mg/(L h) and 0.01–0.030 mol/mol indene, respectively, in the SCB. The hollow fiber membranes in the IHFMB facilitated a more effective biotransformation through reactant sequestration and cell immobilization, with equal contribution at low indene concentrations, albeit a lower contribution by the latter at 6 g/L indene. The performance of the IHFMB was further improved by process optimization and the highest product titer of 487 mg/L, overall volumetric productivity of 24.6 mg/(L h) and molar yield of 0.126 mol/mol indene were achieved with a module comprising 28 membranes (168 cm2/150 mL working volume) at a medium circulation rate of 5 mL/min and aeration rate of 0.5 vvm. Reusability of the membranes has also been successfully demonstrated through 5 successive operations of batch biotransformation.

Keywords
Pharmaceuticals biotransformation; Substrate inhibition; Immersed hollow fiber membrane bioreactor; Pseudomonas putida
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An immersed hollow fiber membrane bioreactor for enhanced biotransformation of indene to cis-indandiol using Pseudomonas putida
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 87, 15 June 2014, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us