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Modified arthroconidial inoculation method for the efficient fermentation of Antrodia camphorata ATCC 200183

Paper ID Volume ID Publish Year Pages File Format Full-Text
3206 157 2014 9 PDF Available
Title
Modified arthroconidial inoculation method for the efficient fermentation of Antrodia camphorata ATCC 200183
Abstract

•A. camphorata arthroconidia produced in submerged culture were used as inoculum.•Environmental and nutritional factors for arthroconidia germination were studied.•The modified arthroconidium-based inoculation is time and labor saving.

Large-scale submerged fermentation (SmF) of Antrodia camphorata usually encounters engineering problems, such as long period and complex fermentation. In this study, a modified inoculation method using arthroconidia sporulated in liquid medium was developed for an efficient large-scale fermentation of A. camphorata ATCC 200183. The optimum environmental and nutritional conditions for arthroconidial germination in the dark control were estimated as follows: temperature, 26 °C; glucose, 20 g/L; soybean peptone, 10 g/L; Mg2+ concentration, 2 mmol/L; and initial pH, 3.5. All the arthroconidia rapidly germinated within 32 h after inoculation under optimal conditions. In the shaking flask, prepared according to the arthroconidial inoculation method, the fermentation period, maximal cell growth rate, and triterpenoid productivity of SmF were 0.6-, 1.7-, and 1.6-fold of those obtained from the culture prepared according to the conventional mycelial inoculation method. Furthermore, efficient fermentation was obtained in a 4000 L fermentor, with a biomass of 7.84 ± 0.20 g/L and a triterpenoid production of 61.21 ± 2.12 mg/L. Therefore, the modified inoculation method can be used in engineering applications with several advantages, including simplicity, good controllability, and high efficiency (shorten the operation time of mycelium-based inoculation from ≥240 h to 168 h).

Keywords
Antrodia camphorata; Filamentous fungi; Inoculation; Optimization; Scale-up; Submerged culture
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Modified arthroconidial inoculation method for the efficient fermentation of Antrodia camphorata ATCC 200183
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 87, 15 June 2014, Pages 41–49
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us