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nDEP-driven cell patterning and bottom-up construction of cell aggregates using a new bioelectronic chip

Paper ID Volume ID Publish Year Pages File Format Full-Text
321 25 2015 8 PDF Available
Title
nDEP-driven cell patterning and bottom-up construction of cell aggregates using a new bioelectronic chip
Abstract

Creating cell aggregates of controlled size and shape and patterning cells on substrates using a bottom-up approach constitutes important challenges for tissue-engineering applications and studies of cell–cell interactions. In this paper, we report nDEP (negative dielectrophoresis) driven assembly of cells as compact aggregates or onto defined areas using a new bioelectronic chip. This chip is composed of a quadripolar electrode array obtained using coplanar electrodes partially covered with a thin, micropatterned PDMS membrane. This thin PDMS layer was coated with poly-l-lysine and played the role of adhesive substrate for cell patterning. For the formation of detachable cell aggregates, the PDMS was not pretreated and cells were simply immobilized into assemblies maintained by cell–cell adhesion after the electric field removal. Cell viability after exposition to DEP buffer was also assessed, as well as cell spreading activity following DEP-driven assembly.

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Keywords
Negative dielectrophoresis; Micro-chip; Cell patterning; PDMS patterning; Poly-lysine coating
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nDEP-driven cell patterning and bottom-up construction of cell aggregates using a new bioelectronic chip
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Publisher
Database: Elsevier - ScienceDirect
Journal: Acta Biomaterialia - Volume 17, 15 April 2015, Pages 107–114
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us