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Intracellular oxidative stress caused by nanoparticles: What do we measure with the dichlorofluorescein assay?

Paper ID Volume ID Publish Year Pages File Format Full-Text
32114 44901 2013 5 PDF Available
Title
Intracellular oxidative stress caused by nanoparticles: What do we measure with the dichlorofluorescein assay?
Abstract

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (149 K)Download as PowerPoint slideHighlights► Proper evaluation of oxidative stress caused by nanoparticle is of crucial importance. ► Dichlorofluorescein (DCF) is the most widespread intracellular assay in nanotoxicology. ► We report widely differing results obtained when testing nanoparticles with DCF assay. ► The suitability of DCF assay for intracellular oxidative stress measurements is discussed.

Keywords
Dichlorofluorescein assay; Cells; Nanoparticles; Reactive oxygen species; Oxidative stressDCFH2, 2′,7′-dichlorodihydrofluorescein diacetate; DCFH2, 2′,7′-dichlorodihydrofluorescein; DCF, 2′,7′-dichlorofluorescein; ROS, reactive oxygen species
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Intracellular oxidative stress caused by nanoparticles: What do we measure with the dichlorofluorescein assay?
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 8, Issue 3, June 2013, Pages 223–227
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us