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Fabrication of metal nanoshell quantum-dot barcodes for biomolecular detection

Paper ID Volume ID Publish Year Pages File Format Full-Text
32115 44901 2013 7 PDF Available
Title
Fabrication of metal nanoshell quantum-dot barcodes for biomolecular detection
Abstract

•Metal nanoshell is grown on the surface of quantum dot barcodes.•This nanoshell improves the stability of the barcodes.•This surface improves the analytical sensitivity barcodes.•This multiplex platform can identify the lethal strain of malaria pathogens.

SummaryQuantum dot (QD) barcoded microbeads are a promising technology for high-throughput biodetection applications. Here we developed QD barcodes of a novel formulation to improve the bead stability, fluorescence consistency, targeting agents loading, and analytical sensitivity as well as to simplify the conjugation process. This novel formulation contains a mixed-polymer system in preparing the barcodes and a seed-mediated strategy to grow metal nanoshells on the surface of QD barcodes. The newly designed barcodes exhibited enhanced stability and a two-order improvement in analytical sensitivity compared with barcodes without any metal coating. This sensitivity enabled the barcodes to be used for multiplexed biosensing, for example, to differentiate the deadly malaria pathogen strain Plasmodium falciparum from the less lethal Plasmodium vivax specie in a single vial. Such improvements in QD barcodes properties will allow this multiplexed detection platform to emerge from academic development into broad practical applications.

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Keywords
Quantum dots; Multiplexing; Metal nanoshell; Biodetection; Malaria; Pathogen
First Page Preview
Fabrication of metal nanoshell quantum-dot barcodes for biomolecular detection
Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 8, Issue 3, June 2013, Pages 228–234
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering