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Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA

Paper ID Volume ID Publish Year Pages File Format Full-Text
32176 44907 2013 19 PDF Available
Title
Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA
Abstract

SummarySensitivity and specificity are two most important factors to take into account for molecule sensing, chemical detection and disease diagnosis. A perfect sensitivity is to reach the level where a single molecule can be detected. An ideal specificity is to reach the level where the substance can be detected in the presence of many contaminants. The rapidly progressing nanopore technology is approaching this threshold. A wide assortment of biomotors and cellular pores in living organisms perform diverse biological functions. The elegant design of these transportation machineries has inspired the development of single molecule detection based on modulations of the individual current blockage events. The dynamic growth of nanotechnology and nanobiotechnology has stimulated rapid advances in the study of nanopore based instrumentation over the last decade, and inspired great interest in sensing of single molecules including ions, nucleotides, enantiomers, drugs, and polymers such as PEG, RNA, DNA, and polypeptides. This sensing technology has been extended to medical diagnostics and third generation high throughput DNA sequencing. This review covers current nanopore detection platforms including both biological pores and solid state counterparts. Several biological nanopores have been studied over the years, but this review will focus on the three best characterized systems including α-hemolysin and MspA, both containing a smaller channel for the detection of single stranded DNA, as well as bacteriophage phi29 DNA packaging motor connector that contains a larger channel for the passing of double stranded DNA. The advantage and disadvantage of each system are compared; their current and potential applications in nanomedicine, biotechnology, and nanotechnology are discussed.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (171 K)Download as PowerPoint slideHighlights► Nanopore based single molecule analysis is currently an area of great interest in many disciplines. ► We review the concept of nanopores as well as types and attributes of various biological and synthetic nanopores. ► We discuss the current and potential applications of nanopores in nanomedicine, biotechnology, and nanotechnology.

Keywords
Bacteriophage phi29; α-Hemolysin; MspA; Solid state pore; Synthetic nanopores; DNA packaging; Nanomotor; Connector; Liposomes; Ion channel; Single channel conductance; Membrane channel; Viral assembly; Stoichiometry quantification; Nanostructure; Bionanot
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Solid-state and biological nanopore for real-time sensing of single chemical and sequencing of DNA
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 8, Issue 1, February 2013, Pages 56–74
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us