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Hydrolysis of lactose in whole milk catalyzed by β-galactosidase from Kluyveromyces fragilis immobilized on chitosan-based matrix

Paper ID Volume ID Publish Year Pages File Format Full-Text
3218 159 2013 11 PDF Available
Title
Hydrolysis of lactose in whole milk catalyzed by β-galactosidase from Kluyveromyces fragilis immobilized on chitosan-based matrix
Abstract

•β-gal from K. fragilis was immobilized on organic supports by different protocols.•β-gal linked on glutaraldehyde-chitosan showed the best catalytic properties.•Glutaraldehyde-chitosan allowed to immobilize high concentration of the enzyme.•The biocatalysts presented high storage, operational and thermal stabilities.•Immobilized β-gal hydrolyzed efficiently lactose in whole milk by batch system.

β-Galactosidase (β-gal) from Kluyveromyces fragilis was immobilized on chitosan-based matrices and agarose beads by different protocols to catalyze the hydrolysis of whole milk lactose in a batch system. The best derivative was obtained by immobilizing the enzyme on chitosan coagulated with KOH and activated with glutaraldehyde, both steps carried out at 50 °C. The immobilization yield and recovered activity were 100%. Maximum activity was achieved at pH 7.0 and 45 °C for both soluble and immobilized β-gal. The maximum β-gal load immobilized on chitosan-glutaraldehyde support was around 21 mg of enzyme per gram of support. Immobilized β-gal was 3–5-fold more stable than the soluble enzyme at 40 and 20 °C, respectively. The biocatalyst retained 80% of its initial activity after 3 months at 10 °C and pH 7.0 in the presence of the cofactors Mn2+ and Mg2+, twice more stable than soluble enzyme. The immobilized biocatalyst was capable to efficiently hydrolyze lactose into the whole milk at 25 °C (above 95% conversion), showing similar performance after four consecutive batches.

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Keywords
Immobilized enzymes; Lactose; Whole milk; Batch processing; β-Galactosidase; Chitosan
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Hydrolysis of lactose in whole milk catalyzed by β-galactosidase from Kluyveromyces fragilis immobilized on chitosan-based matrix
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 81, 15 December 2013, Pages 54–64
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us