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Multiplex targeted in vivo cancer detection using sensitive near-infrared SERS nanotags

Paper ID Volume ID Publish Year Pages File Format Full-Text
32198 44909 2012 9 PDF Available
Title
Multiplex targeted in vivo cancer detection using sensitive near-infrared SERS nanotags
Abstract

SummaryOne of the most promising advantages of surface-enhanced Raman scattering (SERS) technique for in vivo biosensing is the multiplexing potential, which is under explored due to the limited availability of near-infrared Raman reporters. Here, we report the synthesis of multiplexing capable and biocompatible SERS nanotags using highly sensitive novel NIR Raman reporters. Two new NIR Raman reporter molecules, Cy7LA, Cy7.5LA are developed to partner with recently synthesized CyNAMLA-381 reporter to construct SERS nanotags with multiplexing capability. These nanotags possess excellent SERS signal stability over a period of one month. As a proof of concept for multiplex targeted in vivo detection, we successfully demonstrated the simultaneous sensing of cancer in living mouse using these three bioconjugated nanotags. To the best of our knowledge, this is the first real demonstration of in vivo multiplex targeted detection using SERS nanotags. Further, in vivo kinetic study of these nanotags in tumor revealed their excellent sensitivity, stability and tumor specificity. These probes also show rapid clearance from the liver indicating their possible excretion. This validation renders our SERS nanotags as an ultrasensitive in vivo nanoprobe for the detection and imaging of multiple biomarkers for early diagnosis of diseases.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (225 K)Download as PowerPoint slideHighlights► We synthesize three sensitive SERS nanotags with novel NIR Raman reporters. ► These nanotags show excellent stability over a period of one month. ► Multiplex targeted in vivo cancer detection is demonstrated in a living mouse. ► In vivo kinetic monitoring of the SERS nanotags was carried out over several days at tumor and liver location.

Keywords
Raman spectroscopy; NIR Raman reporter; Multiplexing; In vivo cancer detection; SERS nanotags; Nanoparticle kinetics
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Multiplex targeted in vivo cancer detection using sensitive near-infrared SERS nanotags
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 7, Issue 2, April 2012, Pages 85–93
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us