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Elucidating drug resistance properties in scarce cancer stem cells using droplet microarray

Paper ID Volume ID Publish Year Pages File Format Full-Text
32223 44912 2012 6 PDF Available
Title
Elucidating drug resistance properties in scarce cancer stem cells using droplet microarray
Abstract

SummaryThe screening and characterization of cancer stem cells (CSCs) has been challenging due to sample scarcity. We have developed a miniaturized high-content screening (HCS) assay platform to study intra-cellular proteins related to the survival and apoptosis of CSCs with chemotherapeutic treatment. By overcoming critical bottlenecks associated with sample scarcity, we are able to elucidate the drug responses of the side-population (SP) phenotype characteristics of CSCs. This successful miniaturization of HCS assays facilitates the development of cancer drug treatments, and paves the way for nanoliter volume manipulation for cell-based studies and screening.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (234 K)Download as PowerPoint slideHighlights► We have developed miniaturized rinsing assays termed droplet microarray to enable high-content screening of scarce cells. ► The miniaturized cell-based assays were successfully applied to study the drug response of cancer stem cells (CSCs). ► The side-population phenotypes enriched from liver, colon and breast cancer cells showed CSC properties. ► The scarce CSCs were demonstrated to be much more resilient to chemotherapy as compared to their parent cancer cells.

Keywords
Cancer stem cells; High-content screening; Drug resistance
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Elucidating drug resistance properties in scarce cancer stem cells using droplet microarray
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 7, Issue 1, February 2012, Pages 29–34
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us