fulltext.study @t Gmail

Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose

Paper ID Volume ID Publish Year Pages File Format Full-Text
3226 159 2013 10 PDF Available
Title
Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose
Abstract

•Cell mass and productivity were increased compared with glucose-feeding fed-batch.•Great reduction of lactate accumulation and consumption of lactate were realized.•Energy metabolism and utilization of carbon sources were more efficient.•Supply of amino acids and vitamins was optimized for feeding of glucose and galactose.

In order to achieve enhanced cell mass and productivity with less lactate accumulation, a fed-batch culture based on a combined feeding strategy of glucose and galactose was developed. Cell performance was first examined with feeding of galactose alone. While cell growth was improved compared with glucose-feeding culture, cell maintenance was inefficient with rapid lactate depletion and considerable ammonium accumulation. Subsequently, to improve cell maintenance, a combined feeding strategy of glucose and galactose was proposed focusing on optimizing the ratio of glucose to galactose and feeding time. In addition, the compositions of amino acids and vitamins in feeding medium were refined for balanced supply of nutrients. With the combined feeding strategy, the metabolic shift of lactate from production to consumption occurred, but not accompanied by rapid lactate depletion and ammonium production. Furthermore, energy metabolism was more efficient and better utilization of carbon sources was achieved. Compared with the glucose-feeding culture in bioreactor, maximum lactate concentration was reduced by 55%; IVCC and the specific production rate of antibody were increased by 45% and 143%, respectively.

Keywords
CHO cells; Fed-batch culture; Antibody production; Lactate metabolism; Galactose; Process development
First Page Preview
Development of a fed-batch cultivation for antibody-producing cells based on combined feeding strategy of glucose and galactose
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 81, 15 December 2013, Pages 126–135
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us