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Endocytosis and intracellular transport of nanoparticles: Present knowledge and need for future studies

Paper ID Volume ID Publish Year Pages File Format Full-Text
32294 44920 2011 10 PDF Available
Title
Endocytosis and intracellular transport of nanoparticles: Present knowledge and need for future studies
Abstract

SummaryDuring recent years there has been much interest in the use of nanoparticles for in vitro studies as well as for delivery of drugs and contrast agents in animals and humans. To this end it is necessary to increase our understanding of how these particles are taken up and transported within the cells, and to which extent they are metabolized and secreted. In this review we discuss the possibilities, challenges and pitfalls of studying endocytic pathways involved in cellular uptake of nanoparticles. Thus, the use of pharmacological inhibitors, expression of mutated proteins, use of siRNAs and colocalization experiments in such studies are critically evaluated. Although the main focus is on cellular uptake, also aspects of intracellular transport, recycling of nanoparticles to the cell exterior, disturbance of cellular functions, and metabolism of nanoparticles are discussed.

Graphical abstractFigure optionsDownload full-size imageDownload high-quality image (239 K)Download as PowerPoint slideResearch highlights► Studies on the mechanism of interactions between cells and nanoparticles (NPs) must be improved. ► We discuss the possibilities and pitfalls in studies of endocytic pathways followed by NPs. ► We evaluate the use of inhibitors, mutated proteins, siRNAs and colocalization experiments in such studies. ► We discuss intracellular transport, recycling to cell exterior, disturbance of cell functions, and metabolism of NPs. ► Knowledge about cellular fate of NPs is essential to develop nanoparticles for clinical use.

Keywords
CME, clathrin-mediated endocytosis; CIE, clathrin-independent endocytosisNanoparticles; Endocytic mechanisms; Intracellular transport; Pharmacological inhibitors; Toxicity; Metabolism
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Endocytosis and intracellular transport of nanoparticles: Present knowledge and need for future studies
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 6, Issue 2, April 2011, Pages 176–185
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us