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Process engineering of stem cell metabolism for large scale expansion and differentiation in bioreactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
3234 160 2014 9 PDF Available
Title
Process engineering of stem cell metabolism for large scale expansion and differentiation in bioreactors
Abstract

•Stem cells shift from glycolysis to oxidative phosphorylation upon differentiation.•Somatic cells shift from oxidative phosphorylation to glycolysis when reprogramming.•Oxygen and feeding regime modulate stem cell fate decisions in bioreactors.•Understanding stem cell metabolism is critical for process design in bioreactors.

Mesenchymal stem cells (MSCs) and pluripotent stem cells (PSCs) emerge as promising tools for tissue engineering, cell therapy, and drug screening. Their potential use in clinical applications requires the efficient production of differentiated cells at large scale. Glucose, amino acid, and oxygen metabolism play a key role in MSC and PSC expansion and differentiation. This review summarizes recent advances in the understanding of stem cell metabolism for reprogramming, self-renewal, and lineage commitment. From the reported data, efficient expansion of stem cells has been found to rely on glycolysis, while during differentiation stem cells shift their metabolic pathway to oxidative phosphorylation. During reprogramming, the reverse metabolic shift from oxidative phosphorylation to glycolysis has been observed. As a consequence, the demands for glucose and oxygen vary upon different phases of stem cell production. Accurate understanding of stem cell metabolism is critical for the rational design of culture parameters such as oxygen tension and feeding regime in bioreactors towards efficient integrated reprogramming, expansion, and differentiation processes at large scale.

Keywords
Mesenchymal stem cells; Pluripotent stem cells; Metabolism; Process engineering; Bioreactor
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Process engineering of stem cell metabolism for large scale expansion and differentiation in bioreactors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 84, 15 March 2014, Pages 74–82
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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