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Application of Solanum lycopersicum (tomato) hairy roots for production of passivated CdS nanocrystals with quantum dot properties

Paper ID Volume ID Publish Year Pages File Format Full-Text
3239 160 2014 9 PDF Available
Title
Application of Solanum lycopersicum (tomato) hairy roots for production of passivated CdS nanocrystals with quantum dot properties
Abstract

•CdS nanocrystals with quantum dot properties were synthesised using hairy roots.•Exposure to 100 μM Cd elicited nanoparticle synthesis without affecting root growth.•The CdS nanocrystals were passivated and stabilised by phytochelatin peptides.•Nanocrystals were 4–10 nm in size.•Losses during recovery of nanoparticles from the biomass limited product yield.

Semiconductor quantum dot particles have a wide range of applications in medicine, bioassays, computing and photovoltaics. Biological synthesis is an attractive approach for mass production of quantum dots as cells have the capacity to passivate the particles with organic ligands. In this work, hairy roots of Solanum lycopersicum (tomato) were used to produce CdS nanoparticles with quantum dot properties. Treatment of the roots with 100 μM Cd during the mid-growth phase of batch culture elicited cellular responses for Cd detoxification without affecting root growth. A combination of freeze-drying and freeze-thawing of the roots was used to extract Cd from the biomass; anion-exchange chromatography was then applied to selectively remove metal–phytochelatin complexes. Size-fractionation using gel filtration allowed the recovery of phytochelatin-capped Cd- and inorganic sulphide-containing nanoparticles displaying the size and size-dependent optical/electronic properties of CdS quantum dots. At 4–10 nm in diameter, these particles fluoresced at wavelengths corresponding to blue-violet on the colour spectrum and exhibited a high level of photostability with prolonged excitation. Whereas 69% of the Cd extracted from the roots was associated with phytochelatin peptides, the maximum yield of CdS nanocrystals with quantum dot properties was 1.4% of the total Cd taken up into the biomass. This work demonstrates a new culture-based approach for the biosynthesis of metallo-organic semiconductor quantum dots using hairy roots.

Keywords
Cadmium sulphide; Hairy roots; Nanoparticles; Quantum dots; Solanum lycopersicum; Tomato
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Application of Solanum lycopersicum (tomato) hairy roots for production of passivated CdS nanocrystals with quantum dot properties
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 84, 15 March 2014, Pages 36–44
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us