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Development of metal affinity-immobilized liposome chromatography and its basic characteristics

Paper ID Volume ID Publish Year Pages File Format Full-Text
3243 160 2014 8 PDF Available
Title
Development of metal affinity-immobilized liposome chromatography and its basic characteristics
Abstract

•Immobilized liposome chromatography (ILC) was combined with metal affinity.•Ligand-modified liposome immobilized on the gel matrix could adsorb the cupper ion on its surface.•The adsorption of several peptides was dependent on both hydrophobicity and number of His residue.

Metal affinity-immobilized liposome chromatography (MA-ILC) was newly developed as a chromatographic technique to separate and analyze peptides. The MA-ILC matrix gel was first prepared by immobilizing liposomes modified with functional ligands. The functional ligand used to adsorb metal ions was N-hexadecyl iminodiacetic acid (HIDA), which is obtained by attaching a long alkyl chain to an iminodiacetic acid (IDA). Cu(II) ion was first adsorbed on the gel matrix through its complex formation with the HIDA on the surface of the immobilized liposome. Synthetic peptides of various types ranging in size from 5 to 40 residues were then used, and their retention properties on the MA-ILC were evaluated. The retention property of peptides on the MA-ILC by using a usual imidazole elution was compared with the retention property in the case of the immobilized metal affinity chromatography (IMAC) and an immobilized liposome chromatography (ILC). It was found that the retention property of peptides on the MA-ILC has the features of both the IMAC and the ILC; the retention ability of peptides depends on both the number of histidine residues in peptides and the liposome membrane affinity of the peptides. Histidine and tryptophan residues among amino acid residues in peptides indicated a high contribution coefficient for the peptide retention on the MA-ILC, probably due to their metal ion and membrane interaction properties, respectively.

Graphical abstractComparison of recognition site of IMAC, ILC, and MA-ILC.Figure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Metal affinity; Liposome; Immobilization; Chromatography
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Development of metal affinity-immobilized liposome chromatography and its basic characteristics
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 84, 15 March 2014, Pages 66–73
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
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