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Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors

Paper ID Volume ID Publish Year Pages File Format Full-Text
3251 161 2013 8 PDF Available
Title
Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
Abstract

•Influence of KLa on xylanase and β-xylosidase productions by Aspergillus niger in STB and ALB.•Batch fermentations in STB and ALB under a range of kLa for xylanases production.•Comparison of fermentations in STB and ALB for xylanolytic enzymes production.

Oxygenation is an important parameter involved in the design and operation of mixing-sparging bioreactors and it can be analyzed by means of the oxygen mass transfer coefficient (kLa). In this study, batch fermentations in stirred tank bioreactor (STB) and airlift bioreactor (ALB) were operated under a range of kLa values, in attempts to optimize and compare the activities of extracellular xylanase and β-xylosidase synthesized by the fungus Aspergillus niger van Tieghem, using corncob as inducer source. The higher xylanase levels were observed on STB (9000 U l−1; kLa = 30 h−1), while the β-xylosidase production was similar in both bioreactors. However, when the enzymatic activity was compared for the same kLa value (12 h−1) in STB and ALB, the xylanase and β-xylosidase productions were higher in ALB (7000 U l−1 xylanase; 2100 U l−1 β-xylosidase) than STB (4500 U l−1 xylanase; 1800 U l−1 β-xylosidase).

Keywords
Bioreactors; kLa; Xylanase; β-Xylosidase; Corncob; Aspergillus
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Influence of volumetric oxygen transfer coefficient (kLa) on xylanases batch production by Aspergillus niger van Tieghem in stirred tank and internal-loop airlift bioreactors
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 80, 15 November 2013, Pages 19–26
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us