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Simultaneous non-invasive analysis of DNA condensation and stability by two-step QD-FRET

Paper ID Volume ID Publish Year Pages File Format Full-Text
32558 44935 2009 10 PDF Available
Title
Simultaneous non-invasive analysis of DNA condensation and stability by two-step QD-FRET
Abstract

SummaryNanoscale vectors comprised of cationic polymers that condense DNA to form nanocomplexes are promising options for gene transfer. The rational design of more efficient nonviral gene carriers will be possible only with better mechanistic understanding of the critical rate-limiting steps, such as nanocomplex unpacking to release DNA and degradation by nucleases. We present a two-step quantum dot fluorescence resonance energy transfer (two-step QD-FRET) approach to simultaneously and non-invasively analyze DNA condensation and stability. Plasmid DNA, double-labeled with QD (525 nm emission) and nucleic acid dyes, were complexed with Cy5-labeled cationic gene carriers. The QD donor drives energy transfer stepwise through the intermediate nucleic acid dye to the final acceptor Cy5. At least three distinct states of DNA condensation and integrity were distinguished in single particle manner and within cells by quantitative ratiometric analysis of energy transfer efficiencies. This novel two-step QD-FRET method allows for more detailed assessment of the onset of DNA release and degradation simultaneously.

Keywords
Nanocomplex; FRET; Quantum dot; Delivery; Degradation; Nonviral
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Simultaneous non-invasive analysis of DNA condensation and stability by two-step QD-FRET
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Publisher
Database: Elsevier - ScienceDirect
Journal: - Volume 4, Issue 2, April 2009, Pages 125–134
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us