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Mixed culture of Saccharomyces cerevisiae and Acetobacter pasteurianus for acetic acid production

Paper ID Volume ID Publish Year Pages File Format Full-Text
3266 162 2013 5 PDF Available
Title
Mixed culture of Saccharomyces cerevisiae and Acetobacter pasteurianus for acetic acid production
Abstract

•Mixed culture fermentation was used for acetic acid production.•Acetic acid biosynthesis process was controlled at three different periods.•Acetic acid fermentation was optimized by an orthogonal experimental design.•Fed-batch culture strategy was used to increase the yield of acetic acid.

Mixed culture of Saccharomyces cerevisiae and Acetobacter pasteurianus was carried out for high yield of acetic acid. Acetic acid production process was divided into three stages. The first stage was the growth of S. cerevisiae and ethanol production, fermentation temperature and aeration rate were controlled at 32 °C and 0.2 vvm, respectively. The second stage was the co-culture of S. cerevisiae and A. pasteurianus, fermentation temperature and aeration rate were maintained at 34 °C and 0.4 vvm, respectively. The third stage was the growth of A. pasteurianus and production of acetic acid, fermentation temperature and aeration rate were controlled at 32 °C and 0.2 vvm, respectively. Inoculation volume of A. pasteurianus and S. cerevisiae was 16% and 0.06%, respectively. The average acetic acid concentration was 52.51 g/L under these optimum conditions. To enhance acetic acid production, a glucose feeding strategy was subsequently employed. When initial glucose concentration was 90 g/L and 120 g/L glucose was fed twice during fermentation, acetic acid concentration reached 66.0 g/L.

Keywords
Acetic acid; Acetobacter; Yeast; Mixed culture; Orthogonal experimental design; Fed-batch culture
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Mixed culture of Saccharomyces cerevisiae and Acetobacter pasteurianus for acetic acid production
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 79, 15 October 2013, Pages 41–45
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
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Any Questions? feel free to contact us