fulltext.study @t Gmail

Synthetic p-tetrasulphonatocalix[4]arene as novel excipient for lipase-complex

Paper ID Volume ID Publish Year Pages File Format Full-Text
3269 162 2013 6 PDF Available
Title
Synthetic p-tetrasulphonatocalix[4]arene as novel excipient for lipase-complex
Abstract

•Synthesis of new material for excipient complex formation of enzymes.•In the excipient complex formation enzyme retains its activity for a long time as compared to free one.•In the excipient complex form enzyme could be used for variety of purposes.•It is cheap, reusable and less time consuming method.

The present article describes formation of excipient-CRL complex from water soluble calix[4]arene derivative (3 as excipient) and Candida rugosa lipase (CRL), which is proposed as a reusable form of enzyme that is free from steric and diffusion limitations associated with those enzymes immobilized onto porous solid supports. The excipient-CRL could completely hydrolyze 50 mM p-nitrophenyl palmitate (p-NPP) in Tris–HCl buffer at a wide range of temperatures, i.e. 30–80 °C. It is stable under stirred conditions and could be reused multiple times without loss of enzyme activity. It was observed that excipient-CRL complex shows a significant effect on the enzyme activity with an enhancement in thermal stability, while pH and temperature affect the activity of excipient-CRL as well as free CRL. Consequently, the excipient-CRL was found more active than free CRL for the hydrolysis of p-NPP in respect of its reusability.

Keywords
Lipase; Enzyme; Excipient; Hydrolysis; Calixarene; p-Nitrophenyl palmitate
First Page Preview
Synthetic p-tetrasulphonatocalix[4]arene as novel excipient for lipase-complex
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 79, 15 October 2013, Pages 71–76
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us