fulltext.study @t Gmail

Ethanol from laccase-detoxified lignocellulose by the thermotolerant yeast Kluyveromyces marxianus—Effects of steam pretreatment conditions, process configurations and substrate loadings

Paper ID Volume ID Publish Year Pages File Format Full-Text
3272 162 2013 10 PDF Available
Title
Ethanol from laccase-detoxified lignocellulose by the thermotolerant yeast Kluyveromyces marxianus—Effects of steam pretreatment conditions, process configurations and substrate loadings
Abstract

•The use of K. marxianus and laccase is optimized for ethanol from wheat straw.•Steam explosion severity, process configurations and solids loading are studied.•A better yeast performance was observed on slurry obtained at softer conditions.•SSF process showed better results than PSSF at the same conditions.•Laccase led to enhance the yeast fermentation in all tests, specially during SSF.

In our previous work, the ability of laccase enzymes to improve the fermentation performance of the thermotolerant yeast Kluyveromyces marxianus CECT 10875 on steam-exploded wheat straw slurry was demonstrated. As a continuation of this study, the present research evaluates different aspects, including pretreatment conditions, process configurations and substrate loadings, with the aim to proceed towards the use of K. marxianus and laccases for second generation ethanol production. For it, two wheat straw slurries resulting from different steam explosion pretreatment conditions (200 °C, 2.5 min and 220 °C, 2.5 min) were employed at various substrate loadings [5–14% (w/v)] under two process configurations: SSF (simultaneous saccharification and fermentation) and PSSF (presaccharification and simultaneous saccharification and fermentation). The better performance of K. marxianus was observed on the slurry produced at softer conditions. Its lower inhibitors content allowed to increase the total solids loading up to 10% (w/v) in both process configurations, reaching higher ethanol concentrations (12 g/L). Moreover, laccase detoxification improved these results, particularly in SSF processes, increasing the substrate loading up to 12% (w/v) and, consequently, obtaining the highest ethanol concentration (16.7 g/L).

Graphical abstractFigure optionsDownload full-size imageDownload as PowerPoint slide

Keywords
Lignocellulosic ethanol; Kluyveromyces marxianus; Laccase detoxification; Steam explosion; Process configurations; Substrate loadings
First Page Preview
Ethanol from laccase-detoxified lignocellulose by the thermotolerant yeast Kluyveromyces marxianus—Effects of steam pretreatment conditions, process configurations and substrate loadings
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 79, 15 October 2013, Pages 94–103
Authors
, , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us