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Bisulfite as scavenger for enhanced biotechnological production of 3-hydroxypropionaldehyde by Lactobacillus reuteri

Paper ID Volume ID Publish Year Pages File Format Full-Text
3290 162 2013 7 PDF Available
Title
Bisulfite as scavenger for enhanced biotechnological production of 3-hydroxypropionaldehyde by Lactobacillus reuteri
Abstract

•Specific yield of 3-HPA is high with cells from the early stationary phase.•Sulfite and 3-HPA are both toxic for Lactobacillus reuteri.•The 3-HPA-Sulfite adduct is not toxic for L. reuteri.•Use of sulfite to scavenge 3-HPA during production increases specific yield.

Lactobacillus reuteri converts glycerol into 3-hydroxypropionaldehyde (3-HPA), a promising preservative and chemical precursor. Production of 3-HPA by L. reuteri is limited due to the toxicity of 3-HPA to the producing cells. To circumvent this toxicity, the suitability of bisulfite as 3-HPA scavenger during production was assessed. Concentrations of 400 mM 3-HPA or bisulfite caused a viable cell number decrease from 1010 to 107 and 108 cells/ml, respectively, in 1 h. In contrast, the 3-HPA-bisulfite adduct was not toxic for the cells.Cells exhibiting high specific 3-HPA yield in a standardized glycerol conversion test were used to test bisulfite as scavenger during 3-HPA production. Addition of bisulfite (pH 5.8) after 2, 25, and 50 min resulted in conversion of 591 ± 15 mM glycerol within 2 h, similar to the rates under control conditions without bisulfite (501 ± 22 mM). Furthermore, cells from the production with bisulfite were still biological active after 3 consecutive production cycles, converting in total 974 ± 8 mM glycerol in 4 cycles, whereas cells from the control virtually lost their capability to utilize glycerol after two cycles and converted 695.5 ± 3.5 mM.Our results demonstrate the suitability of bisulfite to scavenge 3-HPA to obtain high yields during biotechnological production by the food grade microorganism L. reuteri.

Keywords
In situ product removal; Reuterin; Glycerol
First Page Preview
Bisulfite as scavenger for enhanced biotechnological production of 3-hydroxypropionaldehyde by Lactobacillus reuteri
Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 79, 15 October 2013, Pages 239–245
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering