fulltext.study @t Gmail

Exopolysaccharide production by a marine Pseudoalteromonas sp. strain isolated from Madeira Archipelago ocean sediments

Paper ID Volume ID Publish Year Pages File Format Full-Text
32915 44950 2016 7 PDF Available
Title
Exopolysaccharide production by a marine Pseudoalteromonas sp. strain isolated from Madeira Archipelago ocean sediments
Abstract

•Isolation of a Pseudoalteromonas sp. strain from Madeira Archipelago ocean sediments.•The strain presents autolytic behavior during batch cultivation.•Synthesis of EPS with high uronic acids content.•Fermentation broth develops high viscosity at the end of cultivation.

Exopolysaccharides (EPS) are polymers excreted by some microorganisms with interesting properties and used in many industrial applications. A new Pseudoalteromonas sp. strain, MD12-642, was isolated from marine sediments and cultivated in bioreactor in saline culture medium containing glucose as carbon source. Its ability to produce EPS under saline conditions was demonstrated reaching an EPS production of 4.4 g/L within 17 hours of cultivation, corresponding to a volumetric productivity of 0.25 g/L h, the highest value so far obtained for Pseudoalteromonas sp. strains. The compositional analysis of the EPS revealed the presence of galacturonic acid (41–42 mol%), glucuronic acid (25–26 mol%), rhamnose (16–22 mol%) and glucosamine (12–16 mol%) sugar residues. The polymer presents a high molecular weight (above 1000 kDa). These results encourage the biotechnological exploitation of strain MD12-642 for the production of valuable EPS with unique composition, using saline by-products/wastes as feedstocks.

First Page Preview
Exopolysaccharide production by a marine Pseudoalteromonas sp. strain isolated from Madeira Archipelago ocean sediments
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 33, Issue 4, 25 June 2016, Pages 460–466
Authors
, , , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering