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An efficient enzymatic modification of lily polysaccharide in ionic liquid under ultrasonic irradiation

Paper ID Volume ID Publish Year Pages File Format Full-Text
3296 162 2013 4 PDF Available
Title
An efficient enzymatic modification of lily polysaccharide in ionic liquid under ultrasonic irradiation
Abstract

•Enzymatic acylation of LP in ILs under ultrasonic irradiation was investigated.•Appropriate ultrasonic power can accelerate enzymatic reaction.•Much better enzyme performance under ultrasonic irradiation than magnetic stirring.

A comparative study was made on immobilized Burkholderia cepacia lipase (PSL-C)-catalyzed acylation of lily polysaccharide (LP) with vinyl acetate in the ionic liquid (IL) 1-butyl-3-methylimidazolium tetrafluorobrate ([C4MIm][BF4]) under ultrasonic irradiation and magnetic stirring. The degree of substitution (DS) of the modified LP was used to evaluate the extent of acylation and thus enzymatic activity. The application of ultrasonic irradiation instead of magnetic stirring during acylation gave enhanced DS of the product in a shorter reaction time. The optimization studies also included variation in ultrasonic power, water activity and reaction time. PSL-C displayed higher operational stability and 56.0% of its original activity was maintained after being reused in 8 batches under ultrasonic irradiation. The acylation of LP in [C4MIm][BF4] was shown to be a regioselective one under both conditions, with C6OH being acylated. The results demonstrated that the combined application of ultrasonic irradiation and ILs as a medium may be an efficient approach for enzymatic modification of LP.

Keywords
Lily polysaccharide; Ultrasonic irradiation; Enzyme biocatalysis; Lipase; Immobilized enzymes; Water activity
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An efficient enzymatic modification of lily polysaccharide in ionic liquid under ultrasonic irradiation
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 79, 15 October 2013, Pages 25–28
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us