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Acetic acid inhibition on methanogens in a two-phase anaerobic process

Paper ID Volume ID Publish Year Pages File Format Full-Text
3301 163 2013 7 PDF Available
Title
Acetic acid inhibition on methanogens in a two-phase anaerobic process
Abstract

•We explore the effect of acetic acid on acidogenic phase and methanogenic phase methanogens in a two-phase anaerobic system.•The methanogens were present in both acidogenic phase and methanogenic phase.•The acidogenic phase methanogens could tolerate higher acetic acid concentration than methanogenic phase methanogens.•The methanogenic phase methanogens still tolerated higher undissocaited acetic acid than those in the single stage anaerobic digester.•The high concentration of undissociated acetic acid may still inhibit acidogenic phase and methanogenic phase methanogens.

The inhibitory effect of acetic acid on methanogens in a two-phase anaerobic process was evaluated. The results in this study showed that some methanogens still existed in the acidogenic phase although their dominance in the total microbial community was only 1% compared to 9.6% in the methanogenic phase. The inhibition threshold of acetic acid on acidogenic phase methanogens was, however, higher than that on methanogenic phase methanogens. At pH 6.00, acetic acid inhibition on methanogenic phase methanogens was observed when acetic acid concentration was higher than 1619.47 mg HAc/L although there was no obvious inhibition on acidogenic phase methanogens in the range of 1646.47–2781.19 mg HAc/L. There was also no acetic acid inhibition on acidogenic phase methanogens at pH 5.50, 6.00 and 6.50 in the range of 565.29–2781.19 mg HAc/L. However, for methanogenic phase methanogens, the inhibition was obvious and a second order substrate inhibition model, qs = qmS/[Ks + S + (S2/Ki)], could be adapted to describe the inhibition kinetics and mechanism of undissociated acetic acid on methanogenic phase methanogens. The results showed substrate saturation constant Ks, substrate inhibition constant Ki, and maximum specific utilization rate of acetic acid qm, were 1.66 mg unHAc/L, 145.17 mg unHAc/L, and 3.53 mg HAc/L g MLVSS h, respectively.

Keywords
Aci, initial acetic acid concentration; COD, chemical oxygen demand; CSTR, continuous stirred tank reactor; CT, total acetic acid concentration; F/M, food/microorganism; HAc, acetic acid; HRT, hydraulic retention time; MLVSS, mixed liquor volatile suspend
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 75, 15 June 2013, Pages 1–7
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us