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Transgenic barley overexpressing a cytokinin dehydrogenase gene shows greater tolerance to drought stress

Paper ID Volume ID Publish Year Pages File Format Full-Text
33016 44952 2016 14 PDF Available
Title
Transgenic barley overexpressing a cytokinin dehydrogenase gene shows greater tolerance to drought stress
Abstract

•Stable transgenic barley lines with decreased cytokinin content.•Modification of root architecture by alteration of cytokinin homeostasis.•Greater tolerance to severe drought stress due to better dehydration avoidance.•Higher biomass production when encountering drought stress.•Up-regulation of genes implicated in lignin biosynthesis.

Together with auxins, cytokinins are the main plant hormones involved in many different physiological processes. Given this knowledge, cytokinin levels can be manipulated by genetic modification in order to improve agronomic parameters of cereals in relation to, for example, morphology, yield, and tolerance to various stresses. The barley (Hordeum vulgare) cultivar Golden Promise was transformed using the cytokinin dehydrogenase 1 gene from Arabidopsis thaliana (AtCKX1) under the control of mild root-specific β-glucosidase promoter from maize. Increased cytokinin degradation activity was observed positively to affect the number and length of lateral roots. The impact on morphology depended upon the recombinant protein's subcellular compartmentation. While assumed cytosolic and vacuolar targeting of AtCKX1 had negligible effect on shoot growth, secretion of AtCKX1 protein to the apoplast had a negative effect on development of the aerial part and yield. Upon the application of severe drought stress, all transgenic genotypes maintained higher water content and showed better growth and yield parameters during revitalization. Higher tolerance to drought stress was most caused by altered root morphology resulting in better dehydration avoidance.

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Transgenic barley overexpressing a cytokinin dehydrogenase gene shows greater tolerance to drought stress
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 33, Issue 5, Part B, 25 September 2016, Pages 692–705
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
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Any Questions? feel free to contact us