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Mining the genome of Rhodococcus fascians, a plant growth-promoting bacterium gone astray

Paper ID Volume ID Publish Year Pages File Format Full-Text
33017 44952 2016 12 PDF Available
Title
Mining the genome of Rhodococcus fascians, a plant growth-promoting bacterium gone astray
Abstract

•D188-5, a plasmid-free nonpathogenic derivative of D188, can act as a plant growth promoter.•Biostimulation is achieved by auxin and cytokinin biosynthesis and ACC deaminase.•D188-5 lacks the two major biofertilization mechanisms.•Competitiveness and colonization genes are abundant in the D188-5 genome.

Rhodococcus fascians is a phytopathogenic Gram-positive Actinomycete with a very broad host range encompassing especially dicotyledonous herbaceous perennials, but also some monocots, such as the Liliaceae and, recently, the woody crop pistachio. The pathogenicity of R. fascians strain D188 is known to be encoded by the linear plasmid pFiD188 and to be dictated by its capacity to produce a mixture of cytokinins. Here, we show that D188-5, the nonpathogenic plasmid-free derivative of the wild-type strain D188 actually has a plant growth-promoting effect. With the availability of the genome sequence of R. fascians, the chromosome of strain D188 was mined for putative plant growth-promoting functions and the functionality of some of these activities was tested. This analysis together with previous results suggests that the plant growth-promoting activity of R. fascians is due to production of plant growth modulators, such as auxin and cytokinin, combined with degradation of ethylene through 1-amino-cyclopropane-1-carboxylic acid deaminase. Moreover, R. fascians has several functions that could contribute to efficient colonization and competitiveness, but there is little evidence for a strong impact on plant nutrition. Possibly, the plant growth promotion encoded by the D188 chromosome is imperative for the epiphytic phase of the life cycle of R. fascians and prepares the plant to host the bacteria, thus ensuring proper continuation into the pathogenic phase.

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Mining the genome of Rhodococcus fascians, a plant growth-promoting bacterium gone astray
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 33, Issue 5, Part B, 25 September 2016, Pages 706–717
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us