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Bioresponsive polymers for the detection of bacterial contaminations in platelet concentrates

Paper ID Volume ID Publish Year Pages File Format Full-Text
33023 44953 2014 6 PDF Available
Title
Bioresponsive polymers for the detection of bacterial contaminations in platelet concentrates
Abstract

•We demonstrated that protein based bioresponsive polymers can be used for the detection of pathogens in platelet concentrate.•The backbone of the polymer was designed to be degradable by extracellular proteases released from contaminating pathogens.•A visible colour reaction indicates a contamination and gives a clear yes/no signal.

Bacterial contamination of platelet concentrates (PCs) can lead to fatal transfusion transmitted diseases and is the most abundant infectious risk in transfusion medicine. The storage conditions of PCs provide a good environment for bacterial growth. The detection of these contaminations at an early stage is therefore important to avoid the transfusion of contaminated samples. In this study, bioresponsive polymer (BRP) systems were used for the detection of microorganisms in PCs. The backbone of the polymer consisted of labelled protein (casein), which was demonstrated to be degraded by pure proteases as models and by extracellular enzymes released by contaminating microorganisms. The concomitant colour change was easily visible to the naked eye. To enhance stability, the protein was cross-linked with glycidyl methacrylate (GMA). The cross-linked polymer was easier to handle but was less sensitive than the non-cross-linked material. A contamination of a PC with 10 CFU/mL S. aureus was detectable after 24 hours. The visible colour reaction was quantified as a ΔE value according to the CIELab concept. A ΔE value of 21.8 was already reached after 24 hours. Hence, this simple but effective system could prevent transfusion of a contaminated PC.

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Bioresponsive polymers for the detection of bacterial contaminations in platelet concentrates
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 31, Issue 2, 25 March 2014, Pages 150–155
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us