fulltext.study @t Gmail

Expression of the mammalian peptide hormone obestatin in Trichoderma reesei

Paper ID Volume ID Publish Year Pages File Format Full-Text
33041 44954 2016 8 PDF Available
Title
Expression of the mammalian peptide hormone obestatin in Trichoderma reesei
Abstract

•The potential of T. reesei as an expression host for small peptides has been demonstrated by recombinant expression of obestatin.•Yield of the recombinant obestatin was improved by 18-fold to 5.5 μg/ml through customizing cultivation conditions.•At the time of writing, the 23 a.a obestatin is the smallest mammalian peptide hormone expressed in T. reesei.

The filamentous fungus Trichoderma reesei is an expression host widely exploited for the production of recombinant proteins. However, its capacity for expressing small peptides (<20 kDa) has remained largely uncharted to date. In this work, we have produced the hormone peptide obestatin fused to the hydrophobin I tag (Obe-HFBI), using the T. reesei cellobiohydrolase I core (CBHI) or xylanase 2 (XYN2) pro-region as a carrier and the cbh1 promoter for gene expression, in high protein-low protease producing mutant strains T. reesei Rut-C30 and HEPI. The yield of obestatin was improved from about 300 ng/ml to up to 5.5 μg/ml through adaptive laboratory evolution and modifications to the cultivation strategy, which included adjustments of the type and ratio of carbon and nitrogen sources used in the medium. The successful expression of Obe-HFBI demonstrated the potential of T. reesei as an expression host for small peptides and further enhancement of the recombinant yield through modification of culture conditions.

First Page Preview
Expression of the mammalian peptide hormone obestatin in Trichoderma reesei
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 33, Issue 1, 25 January 2016, Pages 99–106
Authors
, , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us