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Conversion of cheese whey into poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Haloferax mediterranei

Paper ID Volume ID Publish Year Pages File Format Full-Text
33055 44954 2016 7 PDF Available
Title
Conversion of cheese whey into poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Haloferax mediterranei
Abstract

•Haloferax mediterranei was cultivated in cheese whey hydrolyzate for the production of P(3HB-co-3HV).•Medium supplementation with trace elements resulted in improved polymer production.•P(3HB-co-3HV) was extracted from the cells by hypo-osmotic shock.•A high molecular weight biopolymer with low crystallinity was obtained.

Haloferax mediterranei was cultivated in highly saline medium using cheese whey as the substrate for the production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate), P(3HB-co-3HV). Acid hydrolysis provided a simple inexpensive method to obtain a cheese whey hydrolysate that was used for cultivation of H. mediterranei.Batch bioreactor cultivation of H. mediterranei resulted in the production of an active biomass concentration of 7.54 g L−1 with a polymer content of 53%, and a volumetric productivity of 4.04 g L−1 day−1. Supplementation of the cultivation medium with micronutrients favored galactose consumption that was used for polymer synthesis after exhaustion of the available glucose. P(3HB-co-3HV) with a 3-hydroxyvalerate content of 1.5 mol% was extracted from the biomass by hypo-osmotic shock. The polymer presented a molecular mass of 4.4 × 105, with a polydispersity index of 1.5. This work demonstrated the feasibility of using cheese whey for the production of a value-added biopolymer with high volumetric productivity, by using a glucose- and galactose-rich substrate obtained by acid hydrolysis of cheese whey. The use of H. mediterranei as the producing strain avoids the need for strict sterility due to the culture's high salinity requirements and, also, allows for polymer extraction by simply contacting the biomass with water.

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Conversion of cheese whey into poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by Haloferax mediterranei
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 33, Issue 1, 25 January 2016, Pages 224–230
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering