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Silencing UDP-glucose pyrophosphorylase gene in Phaeodactylum tricornutum affects carbon allocation

Paper ID Volume ID Publish Year Pages File Format Full-Text
33057 44954 2016 8 PDF Available
Title
Silencing UDP-glucose pyrophosphorylase gene in Phaeodactylum tricornutum affects carbon allocation
Abstract

•Suppression of UGPase resulted in significant decreases in chrysolaminarin content.•Suppression of UGPase led to increases in lipid synthesis.•UGPase is a rate-limiting enzyme in chrysolaminarin biosynthesis.•UGPase plays an important role and the allocation of carbon.•Ugp is a good candidate gene for allocating overall carbon flux in diatoms.

The effects of the suppression of UDP-glucose pyrophosphorylase (UGPase) on chrysolaminaran biosynthesis and carbon allocation were investigated in Phaeodactylum tricornutum. The 69% decrease in UGPase activity was accompanied by a 4.89 fold reduction in Ugp transcript abundance. Inactivation of UGPase in P. tricornutum led to a significant decrease in chrysolaminaran content and an increase in lipid synthesis. These findings suggest that UGPase is a rate-limiting enzyme and may play an important role in chrysolaminarin biosynthesis and carbon allocation. Our results support a theoretical deduction that Ugp is a good candidate for improving lipid synthesis in diatoms.

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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 33, Issue 1, 25 January 2016, Pages 237–244
Authors
, , , , , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
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