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Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N

Paper ID Volume ID Publish Year Pages File Format Full-Text
3319 164 2013 8 PDF Available
Title
Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N
Abstract

This study aimed to improve the commercial viability of microalgae-based lutein production using an isolated microalga Scenedesmus obliquus CNW-N possessing a high lutein content of over 0.25%. Effective lutein extraction protocols, appropriate storage methods, and purification procedures were developed. Disruption of microalgae cells was most efficient with a bead-beater. The conventional saponification step was modified to reduce the overall extraction time by 24 h. Diethyl ether exhibited the best lutein extraction efficiency. Storage of the lutein extract at low temperature (4 or −20 °C) with antioxidant addition (around 0.01% BHT) can maintain 90% lutein stability after 80 days. Addition of a suitable amount of the antioxidant could promote the stability of lutein extracts under the exposure of light. The protocol developed in this work allows efficient lutein extraction from S. obliquus CNW-N at a lower cost. Further purification was employed to elevate the purity of lutein and its commercial value.

► Microalga Scenedesmus obliquus CNW-N is a suitable candidate for lutein production. ► The developed method shows higher selectivity for extracting lutein from microalgae. ► Optimizing saponfication conditions reduced the operation time from 3 to 6 h. ► Low temperature and antioxidant addition led to higher storage stability for lutein.

Keywords
Microalgae; Scenedesmus obliquus; Lutein; Extraction kinetics; Bioseparations; Purification
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Characterization, extraction and purification of lutein produced by an indigenous microalga Scenedesmus obliquus CNW-N
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 78, 15 September 2013, Pages 24–31
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us