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Transgalactosylation of lactose for synthesis of galacto-oligosaccharides using Kluyveromyces marxianus NCIM 3551

Paper ID Volume ID Publish Year Pages File Format Full-Text
33260 44960 2015 7 PDF Available
Title
Transgalactosylation of lactose for synthesis of galacto-oligosaccharides using Kluyveromyces marxianus NCIM 3551
Abstract

•Synthesis of galacto-oligosaccharides (GOS) using permeabilized whole cells of Kluyveromyces marxianus NCIM 3551.•High yield of GOS with low substrate concentration.•Higher productivity due to shorter reaction time.•Galacto-tetraose as the main reaction product.

Among a number of yeast strains screened for whole cell transgalactosylating activity, Kluyveromyces marxianus NCIM 3551 was found to be most suitable biocatalyst for production of galacto-oligosaccharides (GOS). Cell permeabilization lead to an efficient bioconversion by β-galactosidase resulting in synthesis of GOS. A maximum GOS yield of 36% (w/w) of total sugars was achieved and the products consisted of tri- and tetra-galacto-oligosaccharides. A lactose conversion rate of 80% and productivity of 24 g/L/h was obtained under the optimum conditions at lactose concentration of 20% (w/v), temperature 40°C, pH 6.5 and enzyme units after 3 h of reaction time. Tetrasaccharides were the main component of the reaction mixture. The products were quantitated by HPLC and structurally characterized by mass spectrometry.

First Page Preview
Transgalactosylation of lactose for synthesis of galacto-oligosaccharides using Kluyveromyces marxianus NCIM 3551
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 32, Issue 4, 25 June 2015, Pages 412–418
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering