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Nitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate

Paper ID Volume ID Publish Year Pages File Format Full-Text
33262 44964 2013 6 PDF Available
Title
Nitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate
Abstract

•Type of nitrogen source affects hydrogen production by E. coli.•Ammonium salts have no effect on hydrogen production.•Yeast extract and tryptone positively affect hydrogen production.

The impact of nitrogen source on hydrogen production by Escherichia coli WDHL (ΔhycAΔlacI) strain using cheese whey as a substrate was evaluated. To improve the assimilation of complex proteins such as lactalbumin, we assessed treatment with a protease. Also, five external nitrogen sources were tested: NH4Cl, (NH4)2SO4, urea, yeast extract, and tryptone. The treatments in 120 mL serological bottles with pancreatin 1000 mg/L produced 1.75-fold more hydrogen than the cultures without pancreatin. In the bottle cultures supplemented with yeast extract or tryptone 5 g/L, hydrogen production increased up to 3.2- and 3.5-fold, respectively, whereas inorganic salts and urea had no statistical difference with respect to the control cultures. In 1-L bioreactors, the use of tryptone improved 2.1-fold hydrogen production. Tryptone or yeast extract enable the total consumption of lactose in 40 h, whereas in the control assay the lactose was not completely consumed. Our results demonstrate that it is necessary to select an adequate nitrogen source, which allows both carbon source consumption and high hydrogen production.

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Nitrogen sources impact hydrogen production by Escherichia coli using cheese whey as substrate
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 30, Issue 6, 25 September 2013, Pages 585–590
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us