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Influence of treatment conditions on the oxidation of micropollutants by Trametes versicolor laccase

Paper ID Volume ID Publish Year Pages File Format Full-Text
33288 44964 2013 11 PDF Available
Title
Influence of treatment conditions on the oxidation of micropollutants by Trametes versicolor laccase
Abstract

•Laccase oxidized recalcitrant wastewater micropollutants.•Transformation rates were strongly pH-dependent with optima in acidic conditions.•Optimal temperatures were dependent on micropollutant properties.•A semi-empirical model was developed to predict transformation rates.•Pollutants in mixtures had a strong influence on transformation rates.

Many organic compounds present at low concentrations in municipal wastewater, such as various pharmaceuticals and biocides, are recalcitrant in conventional wastewater treatment plants (WWTPs). To improve their biodegradation, oxidoreductase enzymes such as laccases were tested. The goal was to find optimal conditions for the transformation of two anti-inflammatory pharmaceuticals (diclofenac (DFC) and mefenamic acid (MFA)), one biocide (triclosan (TCN)) and one plastic additive (bisphenol A (BPA)) by Trametes versicolor laccase. Experiments were conducted in spiked solutions at different pH values (from 3 to 9), enzyme concentrations (70–1400 U l−1), reaction times (0–26 hours) and temperatures (10, 25 and 40°C) following a Doehlert experimental design. A semi-empirical model was developed to understand better the combined effects of the four factors and to determine optimal values. This model was able to fit well the experimental data (R2 > 0.97) and showed good predictive ability. All four factors had a significant effect on the micropollutant oxidation with the greatest influence shown by pH. Results for single compounds were different from those obtained for mixtures of micropollutants. For instance, DFC transformation occurred at much higher rates in mixtures under alkaline conditions. Optimal conditions were compound-dependent, but were found to be between pH 4.5 to 6.5 and between 25°C to more than 40°C. A laccase concentration of 730 U l−1 was sufficient to obtain a high removal rate (>90%) of the four individual compounds (range of times: 40 min to 5 hours), showing the potential of laccases to improve biodegradation of environmentally persistent compounds.

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Influence of treatment conditions on the oxidation of micropollutants by Trametes versicolor laccase
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 30, Issue 6, 25 September 2013, Pages 803–813
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us