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Cascade catalysis in membranes with enzyme immobilization for multi-enzymatic conversion of CO2 to methanol

Paper ID Volume ID Publish Year Pages File Format Full-Text
33292 44966 2015 9 PDF Available
Title
Cascade catalysis in membranes with enzyme immobilization for multi-enzymatic conversion of CO2 to methanol
Abstract

•Co-immobilization of three enzymes in a polymeric membrane is conducted.•Enzyme activity was fully retained by fouling-induced immobilization in membrane.•Multi-enzymatic conversion of CO2 to methanol in biocatalytic membrane is evaluated.•Sequential immobilization may be more suitable for multi-enzymatic cascade reaction.•The second reaction catalyzed by formaldehyde dehydrogenase is the cascade bottleneck.

Facile co-immobilization of enzymes is highly desirable for bioconversion methods involving multi-enzymatic cascade reactions. Here we show for the first time that three enzymes can be immobilized in flat-sheet polymeric membranes simultaneously or separately by simple pressure-driven filtration (i.e. by directing membrane fouling formation), without any addition of organic solvent. Such co-immobilization and sequential immobilization systems were examined for the production of methanol from CO2 with formate dehydrogenase (FDH), formaldehyde dehydrogenase (FaldDH) and alcohol dehydrogenase (ADH). Enzyme activity was fully retained by this non-covalent immobilization strategy. The two immobilization systems had similar catalytic efficiencies because the second reaction (formic acid → formaldehyde) catalyzed by FaldDH was found to be the cascade bottleneck (a threshold substrate concentration was required). Moreover, the trade-off between the mitigation of product inhibition and low substrate concentration for the adjacent enzymes probably made the co-immobilization meaningless. Thus, sequential immobilization could be used for multi-enzymatic cascade reactions, as it allowed the operational conditions for each single step to be optimized, not only during the enzyme immobilization but also during the reaction process, and the pressure-driven mass transfer (flow-through mode) could overcome the diffusion resistance between enzymes. This study not only offers a green and facile immobilization method for multi-enzymatic cascade systems, but also reveals the reaction bottleneck and provides possible solutions for the bioconversion of CO2 to methanol.

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Cascade catalysis in membranes with enzyme immobilization for multi-enzymatic conversion of CO2 to methanol
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 32, Issue 3, 25 May 2015, Pages 319–327
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us