fulltext.study @t Gmail

A linker peptide with high affinity towards silica-containing materials

Paper ID Volume ID Publish Year Pages File Format Full-Text
33333 44971 2013 8 PDF Available
Title
A linker peptide with high affinity towards silica-containing materials
Abstract

A peptide sequence with affinity to silica-containing materials was fused to a truncated form of Streptococcus strain G148 Protein G. The resulting recombinant Linker-Protein G (LPG) was produced in Escherichia coli and purified to apparent homogeneity. It displayed high affinity towards two natural clinoptilolite zeolites. The LPG also displayed high binding affinity towards commercial-grade synthetic zeolite, silica and silica-containing materials. A commercial sample of the truncated Protein G and a basic protein, both without the linker, did not bind to natural or synthetic zeolites or silica. We conclude that the zeolite-binding affinity is mediated by the linker peptide sequence. As a consequence, these data may imply that the binding affinity is directed to the SiO2 component rather than to the atomic orientation on the zeolite crystal surface as previously assumed.

► A peptide sequence with affinity to zeolites was fused recombinantly to Protein G’ to produce Linker-Protein G (LPG). ► LPG displayed high binding affinity towards natural and synthetic zeolites. ► We are not aware that the binding affinity of this linker has been previously demonstrated with natural clinoptilolite zeolite. ► The same linker mediates binding affinity to natural or synthetic zeolites.

First Page Preview
A linker peptide with high affinity towards silica-containing materials
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 30, Issue 5, 25 June 2013, Pages 485–492
Authors
, , ,
Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us