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A novel bioactive peptide: assessing its activity over murine neural stem cells and its potential for neural tissue engineering

Paper ID Volume ID Publish Year Pages File Format Full-Text
33342 44971 2013 11 PDF Available
Title
A novel bioactive peptide: assessing its activity over murine neural stem cells and its potential for neural tissue engineering
Abstract

•The Phage Display technique allows us to find peptides binding neural stem cells.•The KLPGWSG peptide binds and enhances per se their neuronal differentiation.•We synthesized a self-assembling peptide functionalized with the KLPGWSG peptide.•We show that this scaffold enhances neural stem cells neuronal differentiation.•We found this effect at a stiffness level matching that of the brain in vivo.

The design of biomimetic scaffolds suitable for cell-based therapies is a fundamental step for the regeneration of the damaged nervous system; indeed growing interest is focusing on the discovery of peptide sequences to modulate the fate of transplanted cells and, in particular, the differentiation outcome of multipotent neural stem cells. By applying the Phage Display technique to murine neural stem cells we isolated a peptide, KLPGWSG, present in proteins involved in both stem cell maintenance and differentiation. We show that KLPGWSG binds molecules expressed on the cell surface of murine adult neural stem cells, thus may potentially be involved in stem cell fate determination. Indeed we demonstrated that this peptide in solution enhances per se cell differentiation toward the neuronal phenotype. Hence, we synthesized two LDLK-12-based self-assembling peptides functionalized with KLPGWSG peptide (KLP and Ac-KLP) and characterized them via atomic force microscopy, rheometry and circular dichroism, obtaining nanostructured hydrogels supporting murine neural stem cells differentiation in vitro. Interestingly, we demonstrated that, when scaffold stiffness is comparable to that of the brain in vivo, the Ac-KLP SAP-based scaffold enhances the neuronal differentiation of neural stem cells. These evidences place both KLPGWSG and the functionalized self-assembling peptide Ac-KLP as promising candidates for, respectively, biomimetic studies and stem cell therapies for nervous regeneration.

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A novel bioactive peptide: assessing its activity over murine neural stem cells and its potential for neural tissue engineering
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 30, Issue 5, 25 June 2013, Pages 552–562
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us