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Direct imaging of DNA motif sequences with encoded nanoparticles

Paper ID Volume ID Publish Year Pages File Format Full-Text
33383 44976 2012 6 PDF Available
Title
Direct imaging of DNA motif sequences with encoded nanoparticles
Abstract

We present a method for encoded tagging and imaging of short nucleic acid motif chains (oligomotifs) using selective hybridization of heterogeneous Au nanoparticles (Au-NP). The resulting encoded NP string is thus representative of the underlying motif sequence. As the NPs are much more massive than the motifs, the motif chain order can be directly observed using scanning electron microscopy. Using this technique we demonstrate direct sequencing of oligomotifs in single DNA molecules consisting of four 100-nt motif chains tagged with four different types of NPs. The method outlined is a precursor for a high density direct sequencing technology.

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Direct imaging of DNA motif sequences with encoded nanoparticles
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 29, Issue 3, 15 February 2012, Pages 279–284
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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