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Inhibitory effect of ectoine on melanogenesis in B16-F0 and A2058 melanoma cell lines

Paper ID Volume ID Publish Year Pages File Format Full-Text
3339 164 2013 7 PDF Available
Title
Inhibitory effect of ectoine on melanogenesis in B16-F0 and A2058 melanoma cell lines
Abstract

Skin injuries, congenital lesions, melasma, Addison's disease and many pigment abnormalities prompt us to search for an effective whitening agent. Ideal whitening agent is a natural compound that can inhibit melanogenesis and has no cytotoxic effects. In a previous study, we have developed an optimum method for the production and characterization of ectoine from a halophilic bacterium isolated from a salt environment in Taiwan was identified as Marinococcus sp. In the present study, we screened the whitening properties of the biosynthesized ectoine using mouse and human melanoma cell lines, B16-F0 and A2058. Here, we examined the cell viabilities of melanoma cells after ectoine treatment at various concentrations up to 500 μM. Also, we addressed the melanin synthesis of melanoma cells after treatment with ectoine. The inhibitory effects of ectoine on tyrosinase activity were assessed in both mushroom tyrosinase and cellular tyrosinase. Furthermore, we investigated the type of inhibition of mushroom tyrosinase using Lineweaver–Burk enzyme kinetic. The melanogenesis-related gene expression (tyrosinase, TRP1, TRP2 and MITF) and their protein secretion were determined by the assays of quantitative real-time PCR and western blots, respectively. Our results demonstrated that ectoine is a safe and effective whitening agent, inhibited melanin synthesis, reduced both mushroom tyrosinase and cellular tyrosinase, and had various inhibitory effects on the expressions of melanogenesis-related genes and secretion of proteins in mouse and human melanoma cell lines. Thus, we suggest that ectoine can serve as a useful and safe new agent in cosmetic and clinical applications.

► Ectoine is a safe, effective and natural whitening agent for cosmetic application. ► Ectoine has no cytotoxicity on melanoma cell lines up to 500 μM. ► Ectoine inhibits melanin synthesis and tyrosinase activity in melanoma cell lines. ► Ectoine has various effects on expressions of melanogenesis-related genes. ► Ectoine has various effects on secretion of melanogenesis-related proteins.

Keywords
DHICA, 5,6-dihydroxyindole-2-carbixylic acid; DMEM, Dulbecco's modified essential medium; D-PBS, Dulbecco's phosphate buffered saline; ERK, extracellular signal-regulated kinase; FBS, fetal bovine serum; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; l-
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Inhibitory effect of ectoine on melanogenesis in B16-F0 and A2058 melanoma cell lines
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 78, 15 September 2013, Pages 163–169
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us