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Asymmetric reduction of a ketone by wet and lyophilized cells of Geotrichum candidum in organic solvents

Paper ID Volume ID Publish Year Pages File Format Full-Text
33393 44976 2012 6 PDF Available
Title
Asymmetric reduction of a ketone by wet and lyophilized cells of Geotrichum candidum in organic solvents
Abstract

Sixteen organic co-solvents were screened for stereoselective reduction of 1-acetonapthone in aqueous media by whole cells of Geotrichum candidum. Benzyl alcohol was found to be a good co-solvent as it afforded a high coversion and reduced deactivation of the cells. Half-lives of the wet and lyophilized whole cell biocatalysts in pure benzyl alcohol were 23.07 and 11.21 hours, respectively. The initial reaction rates at 30°C were 13.1 and 11.0 μmol/min, respectively, for the wet and lyophilized cells. With optimized conditions in a reaction medium containing phosphate buffer and benzyl alcohol (1:1 by vol) with 230 mM 1-acetonapthone, more than 98% and 81% conversion (ee >99%) was achieved in 5 hours with the wet and lyophilized cells, respectively. Both the cell preparations showed maximum conversion at 30°C. A thermodynamic characterization revealed that the wet cells were more thermostable than the lyophilized cells. The calculated half-life of the wet cells at pH 7 was 93 hours, whereas that of the lyophilized cells was 71 hours at the same condition.

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Asymmetric reduction of a ketone by wet and lyophilized cells of Geotrichum candidum in organic solvents
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 29, Issue 3, 15 February 2012, Pages 359–364
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
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Price was $35.95
You save - $31
Price after discount Only $4.95
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