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Synthesis of methotrexate-conjugated gold nanoparticles with enhanced cancer therapeutic effect

Paper ID Volume ID Publish Year Pages File Format Full-Text
3341 164 2013 6 PDF Available
Title
Synthesis of methotrexate-conjugated gold nanoparticles with enhanced cancer therapeutic effect
Abstract

•We synthesized methotrexate-Au NP conjugate as anticancer drug release system.•Methotrexate acts as both a reducing agent and a capping molecule.•MTT and LDH tests showed conjugates exhibit high cytotoxic effects on cancer cells.

We have developed a simple approach to synthesize gold nanoparticle (AuNP) by utilizing the anti-cancer drug, methotrexate (MTX), as both the reducing agent and the capping molecule. MTX-conjugated AuNPs (MTX-AuNPs) of various sizes (∼3 and ∼20 nm) can be obtained in a one-pot synthesis. We utilized transmission electron microscopy, X-ray diffraction, and UV–vis spectroscopy to characterize these nanoparticles, and Fourier-transform infrared (FTIR) spectroscopy and Raman spectroscopy to investigate the chemical interactions between the MTX and AuNPs. To examine the cancer therapeutic effect of these nanoparticles, we applied the lactate dehydrogenase (LDH) and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay tests. The results demonstrated that the MTX-AuNPs of small size (∼3 nm) exhibited considerably high cytotoxic effects in human choriocarcinoma cell lines compared to the effects of equal doses of free MTX; these results suggest that anti-cancer drug-conjugated AuNPs have an enhanced therapeutic effect.

Keywords
Gold nanoparticles; Methotrexate; In vitro; Cancer therapy
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Synthesis of methotrexate-conjugated gold nanoparticles with enhanced cancer therapeutic effect
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 78, 15 September 2013, Pages 175–180
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us