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Multiplex protein detection with DNA readout via mass spectrometry

Paper ID Volume ID Publish Year Pages File Format Full-Text
33414 44977 2013 6 PDF Available
Title
Multiplex protein detection with DNA readout via mass spectrometry
Abstract

Multiplex protein quantification has been constrained by issues of assay specificity, sensitivity and throughput. This research presents a novel approach that overcomes these limitations using antibody–oligonucleotide conjugates for immuno-polymerase chain reaction (immuno-PCR) or proximity ligation, coupled with competitive PCR and MALDI-TOF mass spectrometry. Employing these combinations of technologies, we demonstrate multiplex detection and quantification of up to eight proteins, spanning wide dynamic ranges from femtomolar concentrations, using only microliter sample volumes.

► Integrated platform for parallel protein detection. ► Highly specific and sensitive protein detection. ► Combination of PLA and immune-PCR with competitive PCR and mass spectrometry.

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Multiplex protein detection with DNA readout via mass spectrometry
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 30, Issue 2, 25 January 2013, Pages 153–158
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us