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Production of 2,3-butanediol by a low-acid producing Klebsiella oxytoca NBRF4

Paper ID Volume ID Publish Year Pages File Format Full-Text
33416 44977 2013 7 PDF Available
Title
Production of 2,3-butanediol by a low-acid producing Klebsiella oxytoca NBRF4
Abstract

2,3-Butanediol (2,3-BDO) is a value-added chemical with great potential for the industrial production of synthetic rubber, plastic and solvent. For microbial production of 2,3-BDO, in this study, Klebsiella oxytoca NBRF4 was constructed by chemical mutation and screening against NaBr, NaBrO3 and fluoroacetate. Among metabolic enzymes involved in the production of lactate, acetate and 2,3-BDO, K. oxytoca NBRF4 possessed 1.2 times lower specific activities of lactate dehydrogenase and phosphotransacetylase, and 22% higher specific acetoin reductase activity than the K. oxytoca ATCC43863 control strain. A series of batch fermentations in a defined medium and application of a statistical tool of response surface method led to the determination of optimal culture conditions: 10% dissolved oxygen level, pH 4.3 and 38°C. The actual results of batch fermentation at the optimal conditions using 44 g/L glucose were coincident with the predetermined values: 14.4 g/L 2,3-BDO concentration, 0.32 g/g yield. To increase 2,3-BDO titer, fed-batch fermentation of K. oxytoca NBRF4 was performed by an intermittent feeding of 800 g/L glucose to control its concentration around 5–20 g/L in the culture broth. Finally, 34.2 g/L 2,3-BDO concentration and 0.35 g/g yield were obtained without organic acid production in 70 hours of the fed-batch culture, which were 2.4 and 1.2 times higher than those of the batch fermentation using 44 g/L glucose.

► 2,3-Butanediol (2,3-BDO) is a potent chemical for production of synthetic rubber. ► Klebsiella oxytoca NBRF4 was constructed to have low-acid producing activity. ► Statistical tools were employed to optimize DO level, pH and culture temperature. ► Fed-batch culture of NBRF4 resulted in 34.2 g/L 2,3-BDO without acid formation.

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Production of 2,3-butanediol by a low-acid producing Klebsiella oxytoca NBRF4
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 30, Issue 2, 25 January 2013, Pages 166–172
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us