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Human γD-crystallin aggregation induced by ultraviolet C irradiation is suppressed by resveratrol

Paper ID Volume ID Publish Year Pages File Format Full-Text
3343 164 2013 9 PDF Available
Title
Human γD-crystallin aggregation induced by ultraviolet C irradiation is suppressed by resveratrol
Abstract

•UV-C induced human γD-crystallin aggregation is suppressed by resveratrol.•Resveratrol's anti-aggregating potency relates to its radical scavenging ability.•UV-C irradiation attenuates the sulfhydryl group content in human γD-crystallin.•Addition of resveratrol decreases the UV-C induced reduction in sulfhydryl content.

Human γD-crystallin, a 173-residue protein, is a major protein component of the human eye lens and associated with the development of juvenile and mature-onset cataracts. The study reported here is aimed at examining the effects of resveratrol on human γD-crystallin aggregation and/or precipitation triggered by ultraviolet-C (UV-C) light. Our turbidity, electron microscopy (TEM), and SDS–PAGE results showed that, under UV-C irradiation, resveratrol was able to suppress the formation of human γD-crystallin aggregates and the observed aggregation inhibition was dependent upon the concentration of resveratrol added. Through the use of Ellman reagent, the concentration of sulfhydryl group was observed to decrease upon prolonged UV-C irradiation. Also, there existed a positive correlation between the content of sulfhydryl group in human γD-crystallin and the concentration of resveratrol. Furthermore, we propose that resveratrol's free radical scavenging ability is likely to be correlated with its mechanism of action in inhibiting human γD-crystallin aggregation induced by UV-C irradiation. We believe this work may aid in the development of potential therapeutics for cataract.

Keywords
Resveratrol; Human γD-crystallin; Ultraviolet; Aggregation; Cataract; Free radical
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Human γD-crystallin aggregation induced by ultraviolet C irradiation is suppressed by resveratrol
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 78, 15 September 2013, Pages 189–197
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us