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Intracellular protein production in Trichoderma reesei (Hypocrea jecorina) with hydrophobin fusion technology

Paper ID Volume ID Publish Year Pages File Format Full-Text
33430 44977 2013 7 PDF Available
Title
Intracellular protein production in Trichoderma reesei (Hypocrea jecorina) with hydrophobin fusion technology
Abstract

Insufficient accumulation and the lack of efficient purification methods are the two major bottlenecks hindering the recombinant production of many proteins. Alternative production schemes are urgently needed for proteins that remain challenging to express and purify with conventional techniques. We have found that hydrophobin fusions targeted to endoplasmic reticulum (ER) can enhance the expression of target proteins simultaneously providing means for straightforward purification. Here we show that hydrophobin fusion technology induces formation of large protein bodies in the filamentous fungus Trichoderma reesei. The fusion protein remained soluble in the ER-derived protein bodies. A simple and scalable aqueous two-phase system was demonstrated to purify the hydrophobin fusion protein GFP-HFBI from the complex intracellular extracts with a recovery of up to 62%.

First Page Preview
Intracellular protein production in Trichoderma reesei (Hypocrea jecorina) with hydrophobin fusion technology
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 30, Issue 2, 25 January 2013, Pages 262–268
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering