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CDC19 encoding pyruvate kinase is important for high-temperature tolerance in Saccharomyces cerevisiae

Paper ID Volume ID Publish Year Pages File Format Full-Text
33433 44978 2012 11 PDF Available
Title
CDC19 encoding pyruvate kinase is important for high-temperature tolerance in Saccharomyces cerevisiae
Abstract

Use of thermotolerant strains is a promising way to reduce the cost of maintaining optimum temperatures in the fermentation process. Here we investigated genetically a Saccharomyces cerevisiae strain showing a high-temperature (41 °C) growth (Htg+) phenotype and the result suggested that the Htg+ phenotype of this Htg+ strain is dominant and under the control of most probably six genes, designated HTG1 to HTG6. As compared with a Htg− strain, the Htg+ strain showed a higher survival rate after exposure to heat shock at 48 °C. Moreover, the Htg+ strain exhibited a significantly high content of trehalose when cultured at high temperature and stronger resistance to Congo Red, an agent that interferes with cell wall construction. These results suggest that a strengthened cell wall in combination with increased trehalose accumulation can support growth at high temperature. The gene CDC19, encoding pyruvate kinase, was cloned as the HTG2 gene. The CDC19 allele from the Htg+ strain possessed five base changes in its upstream region, and two base changes resulting in silent mutations in its coding region. Interestingly, the latter base changes are probably responsible for the increased pyruvate kinase activity of the Htg+ strain. The possible mechanism leading to this increased activity and to the Htg+ phenotype, which may lead to the activation of energy metabolism to maintain cellular homeostasis, is discussed.

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CDC19 encoding pyruvate kinase is important for high-temperature tolerance in Saccharomyces cerevisiae
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 29, Issue 2, 15 January 2012, Pages 166–176
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us