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Production of high amounts of 3-hydroxypropionaldehyde from glycerol by Lactobacillus reuteri with strongly increased biocatalyst lifetime and productivity

Paper ID Volume ID Publish Year Pages File Format Full-Text
33439 44978 2012 7 PDF Available
Title
Production of high amounts of 3-hydroxypropionaldehyde from glycerol by Lactobacillus reuteri with strongly increased biocatalyst lifetime and productivity
Abstract

3-Hydroxypropionaldehyde (3HPA) is a promising versatile substance derived from the renewable feedstock glycerol. It is a product of glycerol metabolism in Lactobacillus reuteri. Because of toxic effects, the biotechnological production is poor. In this work the biocatalyst lifetime and product formation could be drastically increased. In the established two-step process already applied, cells are grown in the first step under anaerobic conditions, and in the second step the immobilised or suspended biocatalyst is used for 3HPA-production under strict anaerobic conditions. In the first step it was possible to reach a biomass concentration of 5.5 g CDW/L (OD600 ≈ 23.4). In the second step, normally, 3HPA accumulates to a toxic concentration and the reaction stops in less than 60 min because of the interaction of 3HPA with cell components. To prevent this, the toxic product is bound to the newly found scavenger carbohydrazide to form the hydrazone. For the first time it was possible to recycle the immobilised biocatalyst for at least ten cycles (overall life time > 33 hours) in a repeated batch biotransformation with an overall production of 67 g 3HPA. The optimal pH-value was between 6.8 and 7.2 at an optimal temperature of 40–45°C. In a single batch biotransformation with suspended resting cells it was possible to produce 150 g/L 3HPA as carbohydrazone at an overall productivity of 10.7 g L−1 hours−1. In a single fed-batch biotransformation at 45°C 138 g/L glycerol was converted into 108 g/L 3HPA with an overall productivity of 21.6 g L−1 hours−1. This is the highest 3HPA concentration and productivities reported so far for the microbial production of 3HPA from glycerol.

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Production of high amounts of 3-hydroxypropionaldehyde from glycerol by Lactobacillus reuteri with strongly increased biocatalyst lifetime and productivity
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 29, Issue 2, 15 January 2012, Pages 211–217
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us