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Improved mannan-degrading enzymes’ production by Aspergillus niger through medium optimization

Paper ID Volume ID Publish Year Pages File Format Full-Text
33449 44979 2011 7 PDF Available
Title
Improved mannan-degrading enzymes’ production by Aspergillus niger through medium optimization
Abstract

The effect of different carbon and nitrogen sources on the production of mannan-degrading enzymes, focussing on β-mannanase, by Aspergillus niger was investigated using shake flask culture. The β-mannanase activity obtained during growth of A. niger on guar gum (GG, 1495 nkat mL−1) was much higher than those observed on other carbon substrates, locust bean gum (1148 nkat mL−1), α-cellulose (10.7 nkat mL−1), glucose (8.8 nkat mL−1) and carboxymethylcellulose (4.6 nkat mL−1). For fermentation using GG as a carbon source, bacteriological peptone gave the highest β-mannanase activity (1744 nkat mL−1) followed by peptone from meat (1168 nkat mL−1), yeast extract (817 nkat mL−1), ammonium sulphate (241 nkat mL−1), ammonium nitrate (113 nkat mL−1) and ammonium chloride (99 nkat mL−1) when used as a nitrogen source. The composition of bacteriological peptone and initial pH of the medium were further optimized using response surface methodology (RSM). Medium consisted of 21.3 g L−1 GG and 57 g L−1 peptone with initial culture pH of 5.5 was optimum for β-mannanase production (2063 nkat mL−1) by A. niger. The β-mannanase production obtained in this study using A. niger was significantly higher than those reported in the literature.

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Improved mannan-degrading enzymes’ production by Aspergillus niger through medium optimization
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Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 2, 28 February 2011, Pages 146–152
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
Don't Miss Today's Special Offer
Price was $35.95
You save - $31
Price after discount Only $4.95
100% Money Back Guarantee
Full-text PDF Download
Online Support
Any Questions? feel free to contact us