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Lipase from marine Aspergillus awamori BTMFW032: Production, partial purification and application in oil effluent treatment

Paper ID Volume ID Publish Year Pages File Format Full-Text
33475 44980 2011 12 PDF Available
Title
Lipase from marine Aspergillus awamori BTMFW032: Production, partial purification and application in oil effluent treatment
Abstract

Marine fungus BTMFW032, isolated from seawater and identified as Aspergillus awamori, was observed to produce an extracellular lipase, which could reduce 92% fat and oil content in the effluent laden with oil. In this study, medium for lipase production under submerged fermentation was optimized statistically employing response surface method toward maximal enzyme production. Medium with soyabean meal-0.77% (w/v); (NH4)2SO4-0.1 m; KH2PO4-0.05 m; rice bran oil-2% (v/v); CaCl2-0.05 m; PEG 6000-0.05% (w/v); NaCl-1% (w/v); inoculum-1% (v/v); pH 3.0; incubation temperature 35 °C and incubation period-five days were identified as optimal conditions for maximal lipase production. The time course experiment under optimized condition, after statistical modeling, indicated that enzyme production commenced after 36 hours of incubation and reached a maximum after 96 hours (495.0 U/ml), whereas maximal specific activity of enzyme was recorded at 108 hours (1164.63 U/mg protein). After optimization an overall 4.6-fold increase in lipase production was achieved. Partial purification by (NH4)2SO4 precipitation and ion exchange chromatography resulted in 33.7% final yield. The lipase was noted to have a molecular mass of 90 kDa and optimal activity at pH 7 and 40 °C. Results indicated the scope for potential application of this marine fungal lipase in bioremediation.

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Lipase from marine Aspergillus awamori BTMFW032: Production, partial purification and application in oil effluent treatment
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 6, October 2011, Pages 627–638
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering