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Continuous succinic acid fermentation by Actinobacillus succinogenes

Paper ID Volume ID Publish Year Pages File Format Full-Text
3348 165 2013 7 PDF Available
Title
Continuous succinic acid fermentation by Actinobacillus succinogenes
Abstract

Fermentations were performed in an external recycle bioreactor using CO2 and d-glucose at feed concentrations of 20 and 40 g L−1. Severe biofilm formation prevented kinetic analysis of suspended cell (‘chemostat’) fermentation, while perlite packing enhanced the volumetric productivity by increasing the amount of immobilised cells. The highest productivity of 6.35 g L−1 h−1 was achieved at a dilution rate of 0.56 h−1. A constant succinic acid yield of 0.69 ± 0.02 g/(g of glucose consumed) was obtained and found to be independent of the dilution rate, transient state and extent of biofilm build-up – approximately 56% of the carbon that formed phosphoenolpyruvate ended up as succinate. Byproduct analysis indicated that pyruvate oxidation proceeded solely via the formate-lyase pathway. Cell growth and corresponding biofilm formation were rapid at dilution rates higher than 0.35 h−1 when the product concentrations were low (succinic acid < 10 g L−1), while minimal growth was observed at succinic acid concentrations above this threshold.

Keywords
Continuous fermentation; Succinic acid; Actinobacillus succinogenes; Bioreactors; Biofilms; Glucose
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Publisher
Database: Elsevier - ScienceDirect
Journal: Biochemical Engineering Journal - Volume 73, 15 April 2013, Pages 5–11
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering
Get Full-Text Now
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Price was $35.95
You save - $31
Price after discount Only $4.95
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Full-text PDF Download
Online Support
Any Questions? feel free to contact us