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Statistical optimization of xylitol production from corncob hemicellulose hydrolysate by Candida tropicalis HDY-02 ☆

Paper ID Volume ID Publish Year Pages File Format Full-Text
33480 44980 2011 6 PDF Available
Title
Statistical optimization of xylitol production from corncob hemicellulose hydrolysate by Candida tropicalis HDY-02 ☆
Abstract

The statistical experimental designs were adopted to optimize the culture medium in xylitol production by Candida tropicalis HDY-02 with corncob hemicellulose hydrolysate as substrate. In the first step, Plackett–Burman design was used for screening the important variables. KH2PO4, yeast extract, (NH4)2SO4 and MgSO4·7H2O were found to significantly affect xylitol yield. In the second step, central composite design (CCD) was used to determine the optimum level of each of the significant variables. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The interactive effects of yeast extract and MgSO4·7H2O on xylitol yield of C. tropicalis HDY-02 were determined to be significant. The validation experimental was consistent with the prediction model. The optimum combinations for xylitol yield were 5 g l−1 (NH4)2SO4, 1.3 g l−1 KH2PO4, 4.6 g l−1 yeast extract and 0.6 g l−1 MgSO4·7H2O. Under these optimal conditions, the continuous fed-batch experiments could produce xylitol of 58 g l−1 with a yield of 0.73 g g−1 xylose.

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Statistical optimization of xylitol production from corncob hemicellulose hydrolysate by Candida tropicalis HDY-02 ☆
Publisher
Database: Elsevier - ScienceDirect
Journal: New Biotechnology - Volume 28, Issue 6, October 2011, Pages 673–678
Authors
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Subjects
Physical Sciences and Engineering Chemical Engineering Bioengineering